Analysis of Glutathiones (By Post-column Reaction Method) _ Shodex_ HPLC Columns, Detectors, Standards.pdf

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Overview

Analysis of Glutathiones (By Post-column Reaction Method)

Column : Shodex Asahipak ODP-50 6E (6.0mmI.D. x 250mm)
Eluent : 50mM Sodium phosphate buffer(pH2.5)
Reagent : 50mM o-Phthalaldehyde in CH3OH/200mM Sodium phoshate buffer(pH12.0)/
2-Mercaptoethanol=50/450/1
Flow rate : (eluent); 0.5mL/min, (reagent); 0.3mL/min
Detector : Fluorescnce(Ex. 340nm, Em. 420nm)
Column temp. : 40°C


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