Jasco (LC-4000)

The LC-4000 Series is the latest in a long history of innovative HPLC systems developed by JASCO reaching all the way back to the start of commercial HPLC in the early 1970s. The concept of the integrated LC-4000 series HPLC provides key separation platforms at 50MPa, 70MPa and 130MPa which correspond to conventional HPLC, the increasingly popular Rapid Analysis (RHPLC) and sub 2um UHPLC, respectively.

Each platform is supplied with a dedicated pump and autosampler matched to the operating pressure and all three platforms share common detectors optimized for high-speed 100Hz acquisition and the narrow peak shapes common to both RHPLC and UHPLC.

Advances in Solvent Delivery

For over two decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than the commonly used conventional twin-piston reciprocating design.

Pioneering Optical Design

As a pioneer in optical spectroscopy, JASCO has access to optical designs that few can match. Adopting designs from some of our more powerful spectrometers we have developed a range of HPLC optical detectors with unrivalled performance, like the new class leading FP-4020 fl uorescence detector with S/N of over 2300:1, the latest high-speed refractive index detector with micro cell optimized for RHPLC and UHPLC and a completely revised circular dichroism detector for chiral chromatography.

New Technologies for Method Development

With the range of column technologies becoming ever more diverse – the traditional 5 μm fully porous particle is finally losing ground to some of the newer technologies such as the new superfi cially porous solid core (Coreshell) and the wide variety of sub-2 μm particles, the requirements for method development are becoming more demanding. The LC-4000 series has a number of newly developed accessories to help you make better separations faster, with accessories such as, fl exible and stackable column ovens in a range of sizes to accommodate analytical to preparative columns and multiple columns at every scale and with options for integrated 6 or 10 solvent and column selection valves.

Compact and Easy to Use

Despite the extra power delivered by the LC-4000 series HPLC, the standard footprint is only 300 mm wide, a standard system requires only a very small amount of bench space. For easy user-maintenance, all LC-4000 instruments feature front-access for replacing consumables such as pump components and light sources, even the autosampler consumables such as the syringe parts are easy to access.

For those users who require front-panel control, the LC- 4000 series retains the popular keypad and display (with a backlit LED display as standard). Keypad control can still be confi gured for convenient override when under PC control.

Each LC-4000 module is designed for easy maintenance. Front-panel access to light source and consumables

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The LC-4000 series HPLC analytical and preparative HPLC systems have a number of common features:

• 300mm wide footprint
• Each system component is completely stackable into a modest height
• The system is fully integrated with complete system monitoring
• All user replaceable components are accessible through the front panel
• Vertical leak/drain system to channel away solvent
• Integrated earthquake securing option
• All detectors include 100Hz acquistion with interchangeable flowcells

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JASCO LC-4000 Series Type :

 

Conventional HPLC and RHPLC Analytical Systems up to 70 MPa

The LC-4000 Series 70 MPa system has been designed to future-proof your HPLC requirements. The LC-4000 70 MPa system can be used with conventional 3 and 5 μm particle size analytical columns at typical lower pressures around 10 to 20 MPa (1500 to 3000 psi) and can also be used with smaller particles such as shorter length UHPLC columns and superficially porous (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column.

 

UHPLC Analytical Systems at 130 MPa

Ten years after the new segment of the HPLC market, UHPLC has found a niche for those that need to run a large number of samplers or get results quickly. Pioneering column technology is pushing the particle size even smaller. The near 20,000 psi operation of the LC-4000 130 MPa is designed to take advantage of the separation effi ciency of the very small particles couple with longer and narrower columns. The LC-4000 130 MPa system incorporates many of the same features as the 70 MPa system, but with materials designed to withstand higher pressures.

Preparative HPLC Systems

The LC-4000 series HPLC analytical and preparative HPLC systems have a number of common features

• 300 mm wide footprint
• Each system component is completely stackable into a modest height
• The system is fully integrated with complete system monitoring
• All user replaceable components are accessible through the front panel
• Vertical leak/drain system to channel away solvent
• Integrated earthquake securing option
• All detectors include 100 Hz acquisition with interchangeable fl owcells

The LC-4000 preparative HPLC systems includes three platform options

• Up to 20 mL/min for columns up to 21.2 mm ID
• Up to 50 mL/min for columns up to 30 mm ID
• Up to 120 mL/min for columns up to 50 mm ID

Large column oven for multiple preparative columns and optional automatic column switching Preparative detector options including high fl ow short path length fl ow-cells Open-bed fraction collector with sample trays for tubes, plates or large containers ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different signals

 

A comprehensive range of high performance optical detectors

 

MD-4010, MD-4015 and MD-4017 PDA Detectors

The LC-4000 Series includes three PDA detectors. The MD – 4017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhorse for UV-visible detection in the range 200 to 600 nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution.

The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detector. Making use of a 1024 element photometric diode array, the measurement wavelength range is from 190 nm to 900 nm with a resolution of 1 nm and fast spectrum acquisition rate of up to 100 spectra/sec.

When used with the new temperature controlled SP type semi-micro flow cell the MD-4010 is well suited to the narrower peaks typically found with UHPLC or RHPLC separations. The MD-4010 is fitted with an internal mercury lamp for automatic wavelength calibration.

 

 

UV-4070/4075 UV-Visible Detectors

Using the advanced Czerny-Turner monochromator design with optimization of the optical system, baseline change due to refractive index is minimized. Baseline fluctuation from changes in

environmental temperature is reduced with a temperature-controlled cell, baseline drift caused by temperature fluctuation in the light source is also reduced with a temperature controlled light source.The result is excellent stability with the noise level +/- 0.2×10-5 AU and drift 1×10-4 AU/h. Both detectors offer simultaneous dual-wavelength data acquisition and spectral scanning. User maintenance can be done without removing the module from the system.

Frontpanel access is used for light source replacement and automatic wavelength calibration can be carried out using a mercury lamp. Both detectors  include high speed data acquisition at up to 100 Hz and can be used for conventional HPLC with the standard flow cell and for UHPLC/RHPLC when used with the SP type semi-micro cell.

 

FP-4020/4025 Fluorescence detector

The FP-4020 fl uorescence detector has been designed for the pursuit of extreme sensitivity. Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature-regulated cell body has led to the highest sensitivity with stable detection. These changes have resulted in a giant leap in sensitivity for the FP-4020 to achieve a signal noise ratio of 2300:1 or higher (based on the water Raman peak).

The FP-4025 is an excellent routine fl uorescence detector that has a slightly lower specifi cation with a water Raman S/N of 1400:1 .Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the fl ow cell assembly and light source.

 

Refractive Index detector RI-4030/4035

The Refractive Index detectors include the optical system mounted in a precisely temperature-regulated housing to eliminate the infl uence of changes in ambient temperature. The RI-4030 and 4035 detectors are fully stable just one hour after powering on. The RI-4030 can be used for both analytical and preparative scales. In preparative mode the maximum fl ow rate is 120 ml/min.

The RI-4035 is a dedicated semi-micro RI detector with a micro-volume fl ow cell designed for use with semi-micro GPC analysis using GPC columns with a fi ne pore size. The 100 Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long life time (over 10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035 detectors can be fitted with an optional pressure release safety-valve to prevent damage to the fl ow cell in the event blockage.

 

Circular Dichroism detector CD-4095

The CD-4095 is a unique HPLC detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectrometers. Optically active compounds with a chromophore close to a chiral center absorb differently left and right circularly polarized light, which can be detected with

excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the elution order of d- and l- enantiomers cannot be easily predicted even when separated by a chiral column. The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring their positive and negative peaks.

It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.

 

Chiral detector OR-4090

The OR-4090 Chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds even in the case they do not show absorption bands. The OR-4090 uses a high intensity Hg-Xe arc lamp (150 W) providing a strong output in the UV/Visible region where the largest optical rotations offer the highest sensitivity. The OR-4090 is therefore a general purpose detector suitable for a wide variety of chiral samples.

 

ChromNAV 2.0 The ‘PowerTool’ for HPLC Analysis

 ChromNAV 2.0 (and ChromNAV-CFR 2.0) are JASCO’s next generation CDS developed from the powerful and easy-to-use ChromNAV 1.0 with a host of exciting new features. With a customizable graphical-user-interface (GUI), the user can set-up the system to display only the functions necessary for their application. This latest intuitive GUI allows the user to quickly learn the operation and explore the extensive functionality for data processing. ChromNAV 2.0 is a universal CDS which can be used with any type of separation – HPLC, UHPLC, RHPLC, prep-HPLC, analytical SFC and prep-SFC. ChromNAV can also satisfi y the demands of dedicated analysis and multi-purpose systems.

ChromNAV 2.0 offers powerful system control and data acquisition with options for 2D (or up to 4D) gradient  control and multi-zone oven control  with control of up to 3 column oven for method development. Solvent and column selection control is increased to include up to 10 positions for both. Previously acquired chromatograms can be overlaid for visual comparison with the data currently being acquired, as a quick indicator to the user of current separation performance. Data acquisition and analysis for  a PDA detector are included as standard in ChromNAV.

The fluorescence and UV-visible detectors include spectral scanning as standard, which can be triggered manually, by time or by threshold. A newly added feature of ChromNAV 2.0 is the automatic message that can be emailed to your PC or smartphone to give an update on the progress of the sequence. With the increasing requirements for

Green Energy, the LC-4000 series has the facility not only to power off  and save lamp life at the end of the run, but also a ‘soft-off’ function to automatically power off a module at the end of the sequence.

 

ChromNav 2.0 standard features

• Peak integration and peak identifi cation
• Peak grouping
• Linear and non-linear quantitation
• 3D chromatogram analysis,
• Spectral analysis for UV-visible, Fluorescence and PDA detectors
• Style report generator
• User calculation

 

ChromNAV 2.0 Optional Applications

• ChromNAV CFR for 21 CFR Part 11 compliance and electronic registration of data
• GPC molecular weight dispersion
• Molecular weight dispersion of low molecular weight heparin
• FUMI theory (Function of Mutual Information) for theoretical precision analysis